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Blood was first diluted 1:1 in PBS, and PBMCs were isolated using SepMate-50 separation tubes (STEMCELL Technologies) according to the manufacturers instructions

Aging 277 NickellsR

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It is regularly employed as a primary precursor to S-adenosylmethionine (SAMe) in laboratory studies analyzing cellular transmethylation reactions

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Notably, the ability of PtpA to regulate GPX4 expression or ferroptosis was lost in cells deficient in PRMT6 or cells treated with a PRMT6 inhibitor, demonstrating that PRMT6 is required for Mtb PtpA-induced ferroptosis