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Mechanisms of tumor escape in the context of the T-cell-inflamed and the non-T-cell-inflamed tumor microenvironment

If the redox potential of a thiol/disulfide couple is to be determined, problems already show up with low molecular mass compounds such as GSH or cysteine, since they inactivate all metal electrodes (Jocelyn, E 0 or midpoint potentials at defined conditions (e.g., E m7 at pH 7) of such compounds are usually not determined directly, but estimated by means of the Nernst equation from concentration changes after equilibration with other redox couples of seemingly known standard potential (Rall and Lehninger, E m7 values ranged from 350 to +40 mV depending on the methodology applied (Rost and Rapoport, E m7 which is at present dogmatically accepted, although method sensitivity remains a problem (Van Laer et al., The experimental difficulties to obtain reliable potentials of thiol/disulfide systems prompt further concern to accept these parameters or changes thereof as critical determinants of biological events

Absorption of glutathione from the gastrointestinal tract

D.AhumadaP.CabreraD.ArabJ

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found upregulated expression of miR-93, miR-320, and miR-16 in the serum of patients suffering from diabetic ED by qRT-PCR, suggesting that these miRNAs might be useful for the early diagnosis of diabetic ED (87)