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Due to intrinsic Cpf1 ribonuclease activity that facilitates crRNA processing and an array of donor DNA sequences for homology-directed repair of double-strand breaks generated by Cpf1, the multi-CRISPR/Cpf1 system is more efficient for multiplex deletion of up to four genes (Jimnez et al., 2019a)

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The above analysis resulted in the identification of a suitable refolding buffer (50 mM Tris-HCl pH 8, 100 mM NaCl, 0.4 M arginine, 3 mM -ME)

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Linking early-life NMDAR hypofunction and oxidative stress in schizophrenia pathogenesis