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A solution containing 1.75 mg/ml fibrinogen and 0.9 g/mL FXIIIa in 50 mM Tris HCl buffer of pH 7.4 containing 10 mM CaCl 2 was incubated with different concentrations of EA (5 5000 M), and then clotted in the presence of human -thrombin (1.25 g/mL)

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Dark spots or hyperpigmentation, including those left behind after acne heals (called post-inflammatory hyperpigmentation), are caused by excess melanin