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LCMS metabolomics analysis Sample preparation HCT116s cells (1 10 5 ) treated with or without 5 M 5FU were cultured in DMEM for 24 h and 72 h or supplemented with media containing uniformly labelled 13 C 6 -glucose (25 mM) for 6 h before sampling

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This term assumes that M1 macrophages can convert into M2 macrophages in response to the anti-M1 agent, which reduces the pro-inflammatory activity of M1 macrophages 25,27

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