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Description

While simply adjusting vitamin levels by increasing intake of the foods or supplements you need can help alleviate symptoms, including fatigue, a clinical diagnosis of B12 deficiency is necessary to get the shots

Indian J Med Res (2008) 128(6):6913

After the centrifuge, digestion was performed in 0.05% trypsin-EDTA (T4174, Sigma-Aldrich, MO, USA) for 5 min at 37 C and stopped by adding an equal volume of trypsin inhibitor (T6522, Sigma-Aldrich) in phosphate-buffered saline (PBS)