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2 Transfer to a -80C freezer for 24 hours

Fukutomi T, Takagi K, Mizushima T et al (2014) Kinetic, thermodynamic, and structural characterizations of the association between Nrf2-DLGex degron and keap1

While GPX3 localized mainly to the basement membrane of the bronchial epithelium and endothelium in healthy mouse and human tissue, we found localization also to the interstitial ECM in fibrotic tissue compartments