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Binding induces autophosphorylation of the intracellular kinase domain on tyrosines 1131/1135/1136, recruits substrates IRS-1, IRS-2 and Shc, and activates two major signaling pathways: the PI3K-AKT-mTOR pathway (protein anabolism, cell growth, apoptosis inhibition) and the Ras-Raf-MEK-ERK pathway (cell proliferation, growth gene transcription)

Protein samples were separated by SDS-PAGE using 4%12% polyacrylamide gels (Bio-Rad)

J Hepatol 33: 247253 Palm W, Park Y, Wright K, Pavlova NN, Tuveson DA, Thompson CB (2015) The utilization of extracellular proteins as nutrients is suppressed by mTORC1

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Piechocki, J