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The bacterial cultures were pelleted for 15 minutes at 4000 x g at 4 C and stored at 20 C until purification

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Next, Np and pNp were buffer exchanged to 20 mM TrisHCl pH 7.5, 150 mM sodium chloride to study intermolecular disulfide formation, which was verified by 20% SDSPAGE analysis at pH 2.5 and pH 7.5 in non-reducing conditions

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This HIV-1 specificity of the suicide vector system was also supported by the observation that iRFP expression was exclusively observed in cells that were also positive for HIV-1/GFP in TNF- treated Ad-iRFP control virus transduced cells (Fig