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At the end of incubation, the protein was separated from the reaction mixture by ultra-filtration (5 kDa cutoff, Vivaspin 500, Sartorius, Germany) and the filtrate was analyzed for the presence of metals after a complete oxidation of the remaining thiols (10 h at 37 C) by adding 100 M PAR, as described above

This selectivity was documented in the foundational Raun et al

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MK-677 is not technically a peptide, but it is included in peptide conversations because it affects growth hormone and IGF-1 signaling

Requirement for an -keto acid or an amino acid oxidase for activity and identity with soluble glutamine transaminase K