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The compound is designed to be included in well-defined experimental frameworks managed by trained laboratory personnel

doi: 10.3389/fneur.2020.00088 Received 19 September 2019 Accepted 24 January 2020 Published 14 February 2020 Volume 11 - 2020 Edited by Pamela Ann McCombe, University of Queensland, Australia Reviewed by Ester Moral, Hospital of Sant Joan Desp Moiss Broggi, Spain

Discussion The original method faced several challenges, including the need for strong acids to terminate the enzymatic reaction, which could be hazardous and affect the accuracy of the measurement

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A solution that stays cloudy or shows visible particulates after adequate swirling time usually points to one of a few causes: incomplete dissolution that just needs more time, an unsuitable solvent for that particular compound, or degradation that occurred somewhere earlier in the handling process