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ACS Appl Mater interfaces

While both are powerful antioxidants that play crucial roles in protecting your cells from oxidative stress, they work in fundamentally different ways

Xiong C, Ling H, Hao Q, Zhou X

Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h

( D ) Representative Western blot of intracellular and secreted type 2 signature markers following SLC7A11 chemical inhibition with erastin for 24 hours ( n = 34)