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Experimentally, the presence of persister cells in Candida biofilms is most often inferred from biphasic killing curves: when mature biofilms are challenged with high concentrations of a fungicidal agent, a rapid initial kill reduces the majority of the population but is followed by a plateau or much slower kill rate in which a small surviving fraction persists despite prolonged exposure (Lewis, 2010

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Cosgrove BD, Gilbert PM, Porpiglia E, Mourkioti F, Lee SP, Corbel SY, Llewellyn ME, Delp SL, Blau HM