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This is often due to limited dietary intake, malabsorption, medical conditions, or the use of B12-depleting medications ( Vitamin B12 deficiency is determined as follows ( Because the ability to absorb B12 from food declines with age, deficiency is more common in older adults

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After subsequent washing, 50l/well of 1:1000 diluted streptavidin-ALP (Mabtech) was added, incubated for 1 hour, and then developed with 50 l/well of AP Conjugate sub kit (BioRad) for 510 minutes

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