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After drug treatments, the cells were fixed with 4% paraformaldehyde for 20 min and infiltrated with 0.2% Triton X-100 for 15 min, followed by blocking in donkey serum for 30 min and incubating overnight at 4C with primary antibody (GPX4, rabbit, ab125066, 1:200, Abcam) in PBS

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LIPUS treatment can improve the regeneration of nerve fibers reaching the target muscles, so it can prevent the irreversible muscle atrophy

Intravenous therapy with this compound would theoretically be safe and effective, although the large quantities required to restore normal glutathione homeostasis make this approach fiscally and medically impractical

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