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Transmission electron microscopy revealed mitochondrial membrane rupture, fragmentation, and mitochondrial cristae fragmentation and disappearance

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After incubation of the supernatants with Escherichia coli NO 3 reductase (37, 30 min), 1 ml of Griess reagent (0.5% naphthylenediamine dihydrochloride, 5% sulfonilamide, 25% H 3 PO 4 ) was added

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