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This ensures the injection is placed correctly in the muscle and minimizes the risk of infection

Thanks to its vitamin E content it also has some antioxidant properties

Sample size was evaluated using Robin Ristls sample size calculator (Medical University of Vienna, for two groups with unequal sample sizes, with mean difference 1.5, standard deviation of 0.6, significance level 0.05, and power 0.8

We likewise purified Mer3 from insect cells as previously described 32 , and demonstrated that both samples were free of detectable nucleic acid contamination as determined by the ratio of the absorbance at 260 nm and 280 nm in size exclusion chromatography (Supplementary Fig

The sample was diluted to 50 mM NaCl and cleared through IEX, followed by SEC using eGFP-CRBN SEC buffer (25 mM Hepes/NaOH pH7.4, 200 mM NaCl, 2 mM TCEP)

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