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B3 An amount of 450 mL of the suspension (step B2) was mixed with 450 mL of fresh DMEM +10 g matrix/100 mL sterile hydroalcoholic solution (90% H 2 0/10% EtOH)

4-HNE protein adducts were detected by an anti 4-HNE protein adduct antibody (1:2000, AB5605, Millipore) in Tris-buffer saline with 5% skim milk, and ECL (GE Healthcare)

What If Water Retention Becomes Severe Enough to Interfere with Data Collection

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