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Cells were cultured and treated in a 37 C humidified incubator gassed with 5% CO 2 and 90% N 2 so as to achieve bone marrow level hypoxia of 5% O 2 or alternatively room air without N 2 to achieve 20% O 2

However, for patients identified preoperatively with severe malnutrition or a high inflammatory state, a more aggressive nutritional strategy may be required, such as early combination with parenteral nutrition (PN) to ensure adequate energy and protein supply, or more cautious selection of feeding tube routes to balance the benefits of enteral nutrition with the risk of aspiration (120)

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This positions GHK-Cu as a model substrate for studying small-peptide control of large gene-network programs.[9] Redox-Silenced Copper Delivery Studies Bioinorganic chemistry investigations use GHK-Cu as a model carrier for delivering Cu(II) to cells without triggering Fenton-driven hydroxyl-radical generation