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10.7150/thno.43577 20 ChettyA.CaoG

Teixeira et al

8th edition, January 2020 OptiPrep Application Sheet C02 Preparation of gradients for cells 1 Discontinuous gradients 1a Overlayering technique The most widely used method for producing discontinuous gradients is to start with the densest solution and layer solutions of successively lower densities on top using some form of pipette or syringe