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The cells were then lysed with 2% digitonin in PBS and the protein concentration was determined using the BCA assay kit

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For that reason, women who are pregnant or planning a pregnancy shouldnt take excessive doses of vitamin A

The immunofluorescence data can be found at: Further enquiries can be directed to the corresponding author

There are different subcellular isoforms of each of the antioxidant enzymes and each isoform differentially responds to different stress and developmental cues

Ascorbic acid (1 mL) was added to a final concentration of 300 M