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Briefly, 0.1 g leaves were extracted with 10 mL of 10% trichloroacetic acid (TCA) and then centrifuged at 10,000g for 20 min

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All marsupial species and monotremes tested have been able to metabolise glucose to lactate and glucose appears to be a good substrate for erythrocyte energy production in native animals

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NAPstars specifically respond to dynamic NADP redox changes in vitro To further test the dynamic and specific responsiveness of NAPstars to changes in NADP redox status, we used recombinant NAPstar probes to monitor NADP dynamics in enzyme assays in vitro (Supplementary Fig