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piGEM009 is a multi-gene expression vector without any transit peptides, while piGEM011 is a multi-gene expression vector with three peptides

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V., & Kozhevnikova, A

Using this codeset, we analyzed gene expression in three settings of mTORC1 activation: 1) wild-type MEFs stimulated with insulin in the presence or absence of rapamycin, 2) growth factor-independent activation of mTORC1 via genetic loss of the TSC protein complex in Tsc2 -/- MEFs, and 3) Tsc2 -/- MEFs with siRNA-mediated knockdown of ATF4 (Figure 2Afigure supplement 2A and Source data 2)

TICs maintain lower intracellular ROS levels than other cancer cells within the tumor, primarily through the accumulation of ROS scavengers like glutathione and the overexpression of Gclm and glutathione synthetase, the enzymes necessary for its synthesis, as shown in breast and liver cancer stem cells [18, 20]

For human fibroblasts, cells were fixed with methanol/acetone (1:1) for 15 min, permeabilized in 0.1% (v/v) Triton X-100 for 15 min and incubated with primary antibodies overnight at 4 C