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The expressed GST genes are distributed across various classes, with a particularly strong representation of the theta class ( GSTT3, n = 41 , Supplementary Dataset 1 )

Notably, animal models have demonstrated that transferring dysbiotic microbiota from CFS/ME patients to germ-free mice can induce fatigue-like behaviors, supporting a causal role for the microbiome in disease pathogenesis (Bansal et al., 2025)

These finding highlights the potential of stress management in treating abdominal pain in IBS-C

Antibodies were affinity-purified using either the peptides linked to SulfoLink beads (Pierce) or the Ascaris proteins (without GST) linked to a mix of Affigel-10/15 (BioRad)

116, no

The protocol number is IAEC/MIET/2022/84