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The expression of SR-BI on macrophages post different treatments was evaluated by immunofluorescence staining using anti-SR-BI antibody (incubated overnight at 4 C) and Alexa Fluor 488-conjugated secondary antibody (incubated for 1 h at room temperature)

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The membrane was blocked in blocking buffer (5% BSA in 1TBST, Tris-buffered saline and Tween-20) and then incubated with primary antibodies overnight at 4 C