glutathione sepharose column sigma Sepharose™ 4B Cytiva 17-0756-04, pack of 300 mL such as native glutathione S Millipore Glutathione−Agarose, set of 3

Vojnov et al., 2010

Scientists have attached an acetyl group (COCH3) directly to the sulfur atom of the cysteine molecule within the glutathione structure

Total GSH is measured at an absorbance of 412 nm as the oxidized GSSG is reduced to two GSHs by NADPH, Glutathione reductase, and the reduced GSH and GSH present in the existing sample react with DTNB to form yellow TNB

Proximity ligation assay Cells were grown on glass cover slips for 36 h, fixed with 3.7% paraformaldehyde for 10 min, and permeabilized with 0.1% Triton X-100 in PBS for 5 min

Glutathione Injection vs Oral Spray 1

siRNA knockdowns Cells were transfected with 20 nM of the indicated siRNAs using Opti-MEM (Thermo, 31985062) and RNAimax (Thermo, 13778150) according to the manufacturers protocol