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To directly test the ability of SNO-CoA to inhibit PDHC, we incubated purified PDHC with SNO-CoA without additional CoA or other NO donors

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[DOI] [PubMed] [Google Scholar] Liu JJ, Lin SH, Xu PL, Wang XJ, Bai JG

Then, the free energy released after ATP conversion to ADP and orthophosphate can be used to accumulate the transported substrates, controlling their movement between cellular compartments or intracellular/extracellular environments by modification of protein conformation