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Lysates were clarified for 10 min at 8,000 g , 1% input samples were reserved, and supernatant was used for immunoprecipitation with Diagenode Protein A-coated Magnetic beads ChIPseq grade (Diagenode, C03010020-660) and H3K3me3 monoclonal antibody (Cell Signaling Technologies, 4909) with 0.1% BSA (Sigma, 10735094001)

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Development buffer (1mg/ml 4-nitrophenylphosphate in diethanolamine buffer) was added, and optical density was measured at 405nm using a BioTeK Microplate Reader

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Tracking changes in SARS-CoV-2 spike: evidence that D614G increases infectivity of the COVID-19 virus