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The resulting BAM files had PCR duplicates, mitochondrial reads, and ENCODE blacklist 107 features removed before further analysis using Picard v3.4.0 (Broad Institute, 2019), samtools v1.13 93 , and bedtools v2.30.0 108 , respectively

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Importantly, adequate training and thoughtfulness to details is important to mitigate inadvertent adverse effects on animal health and confounded experimental outcomes when testing the intranasal method of administration [193]

Science (2006) 312(5780):16503

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