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In particular, miR-16 was reported to be downregulated in osteosarcoma cell lines and tissues (77)

The NO release capability of HMO was further assessed using the Griess reagent method

If its not needed, then stop it or reduce the dose

Glutathione S-transferase (GST) activity was assessed by adding 10 L of the tissue supernatant to a mixture of 10 L of GSH (5 mM) and 10 L of 2,4-dinitrobenzene (CDNB) (1 mM) in 100 L of phosphate buffer, recording at 340 nm for 5 min, and the activity was expressed as moles of CDNB conjugated/min/mg of protein (Al Kury et al., 2019)

IGF-1 DES has a shorter half-life and more localized effect, which means it carries less systemic risk but more site-specific irritation

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