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siRNA-mediated dynamin knockdown siRNA transfection The neurons were co-transfected with 50 nM dynamin 1, 2, and 3 siRNA constructs (previously described in 65 ) or with non-targeting control siRNA (ON-TARGETplus Non-targeting Control Pool

(eds) Drug Discovery from Mother Nature

85 On the other hand, the increase in nitrosylation of Cys166 and Cys131 in the ER thiol oxidase Ero1 leads to a decrease in oxidase activity with aging, stimulating ER reductive stress