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EpiSCs were dissociated with Accutase into single cells and seeded at 10,000 cells per well in a 12-well plate coated with FBS in N2B27 + FAX medium, including Y27632 (5 M)

Inhibitor21 and inhibitor1 are specific and respective inhibitors of APT1 and APT2, respectively [380]

This should be considered as pyroglutamic acidosis until proven otherwise

Since a U-100 syringe has 100 units per mL, each syringe unit equals 50 mcg of total blend

ROS mediated opening of Ca 2+ dependent mitochondrial permeability transition (MPT) pore results in ATP depletion, ultimately leading to necrosis (36, 41)

What markers will be monitored during treatment