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The difference in chemical structure between this peptide and the original IGF-1 is the substitution of arginine at the third position, which significantly modifies the compound

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3) For large volume of supernatant, concentrate the proteins by ammonium sulphate precipitation, dialyze the dissolved protein solution against 1 PBS, and then apply the solution onto the GSH column

The arteries were cleaned of connective tissue and cut into transversal segments (23 mm)

Poster: Elucidating the putative oxygen sensing protein