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Researchers measured several oxidative stress markers and antioxidant enzymes, including GPx, in the blood serum of these cats

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Subsequently, the proteins were transferred onto PVDF membranes, which were probed with specific primary antibodies

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Chromatins were sonicated to 2001000 bp and immunoprecipitated with antibodies against NRF2, H3K4Me3, and H3K9Ac and determined with gene-specific primers (Supplementary Table 1)

Another study [21] using the modified Short Form McGill Pain Questionnaire showed a significant pain reduction at week three of ALC treatment compared with baseline