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Immunofluorescence and fluorescence complementation For immunofluorescence of cultured cells, the cells were fixed with 4% paraformaldehyde (PFA) for 15 min at room temperature, permeabilized with 0.1% TritonX-100 diluted in PBS at room temperature for 5 min, blocked with 10% FBS diluted with PBS for 1 h and primary antibody incubation for 1 h

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In neurodegenerative diseases and in aging they organize the response to OS and damage to DNA and are related to the functionality of the respiratory machinery and the production of ROS in different tissues (Huang et al., 2010)

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