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100 L of beads resuspension was incubated with 5 g of sucA-AMP (470 nM) and either no nucleotide, 1:1 molar ratio nucleotide (470 nM), 1:5 molar ratio nucleotide (2.35 M), or 1:25 molar ratio nucleotide (11.75 M)

In all qPCR experiments, 18s RNA expression was used to normalize gene expression within each tissue sample that was processed in triplicate

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These individuals may experience abrupt elevations in alanine aminotransferase (ALT) levels exceeding 1,000 U/L within 14 months of use, often accompanied by autoimmune hepatitis-like features

doi: 10.1016/j.freeradbiomed.2011.09.030 24 KerksickC.WilloughbyD

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