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The resulting pellets were resuspended by gentle shaking overnight at 4 C in MNT buffer (30 mM 2-( N -morpholino)ethanesulfonic acid, 100 mM NaCl, 20 mM Tris-HCl, pH = 7.4)

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Under these conditions, strain growth was limited, achieving a maximum DCW of only 27 g/L after 68 h of cultivation (Fig