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Vitamin E metabolites have also shown promise in the context of wound healing

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Materials and Methods Cell Culture and Drug Treatments HCM, C2C12 and HUVEC were cultured in high-glucose DMEM (Gibco BRL, USA) supplemented with 5% fetal bovine serum (Gibco BRL, USA) without antibiotics in acceptable conditions (5% CO 2 , 37C)

Growth factor concentration varies per session

Proper technique, correct dilution volume, and refrigerated storage ensure potency and dosing accuracy over a 28-day shelf life