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Coimmunoprecipitation (co-IP) assay and immunoblotting Striatum tissues and cells were lysed using RIPA lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, and 0.5% NP-40) containing protease and phosphatase inhibitors for 1 h on ice and sonicated 3 times using a Sonics VC750 ultrasonic processor

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The Impact of Microbiota on Brain and Behavior: mechanisms & Therapeutic Potential[M] M

Author affiliations 1 Indian Institute of Science Education and Research, Mohali, Punjab, India, 2 DBT- Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, India, Acknowledgement The work was supported by a grant-in-aid project from the Department of Biotechnology (BT/PR39040/BRB/10/1882/2020) to AKB and a DBT-Wellcome Trust India Alliance Senior Fellowship (IA/S/21/2/505922) to SL

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