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mRNA library preparation was performed using Illumina Stranded mRNA Prep, Ligation (Illumina, #20040534) and TruSeq Stranded mRNA Library Prep (Illumina, #20020594) kits on the King Fisher FLEX system (Thermo Fisher Scientific, #18-5400620)

L., et al., Circadian Clocks Regulate Cardiac Arrhythmia Susceptibility, Repolarization, and Ion Channels, Current Opinion in Pharmacology 57 (2021): 1320

doi: 10.1016/j.tibs.2024.12.009 188 WuCLiuYLiuWZouTLuSZhuCet al

Identified cellular targets through which PKA activation produces a bronchodilator effect include: MLCK: Phosphorylation of myosin light chain kinase (MLCK) by cAMP-dependent protein kinase (PKA) decreases the affinity of the kinase for Ca2+ and calmodulin , resulting in a decrease in MLCK activity

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The specific screening procedure is summarized in Figure 1