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For real-time PCR, RNA was extracted using RNAStat60 (Tel-Test) and treated with RQ1 DNAse (Promega, no

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However, the presence of lactyl-CoA at physiological concentrations has not yet been directly demonstrated, and this pathway remains under debate [73]

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Gclc is required for T cell-independent and -dependent B cell immune responses Next, we aimed to understand how the metabolic perturbations caused by B cell-specific Gclc ablation impinge on immune responses and in particular how the impaired mitochondrial metabolism affects the antibody response