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Fusion primers with dual-indexed PCR adapters were used to generate the construct library, and short fragments were removed with AMPure beads

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Meaningful fat loss indicators include measurable changes in body composition, increased energy during fasted states, and visible changes in stubborn fat areas

However, the largest Phase IIb trial involving 536 subjects failed to demonstrate statistically significant weight loss compared to placebo at the primary 12-week endpoint, leading Metabolic Pharmaceuticals to terminate development in 2007