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Studies have demonstrated that propionate is efficiently utilized by the liver as a gluconeogenic substrate, while acetate participates in hepatic de novo lipogenesis (DNL) (56)

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The reaction was then cooled to 0 C and quenched by addition of a saturated aqueous solution of ammonium chloride (3 mL)

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This reduces the "work" your digestive system and kidneys have to do to process unabsorbed material

For flow cytometric analysis of aortic endothelial cells, digestion buffer consisted of 1X PBS containing DNase I (250 U/ml) and collagenase IV (2500 U/ml, LS004212, CellSystems, Troisdorf, Germany) and aortas were digested for 40 min under agitation at 37C