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The glutathione reductase enzyme utilizes FAD and NADPH as cofactors

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In brief, plated cells were incubated for 30 min at 37 C, 5% CO 2 in the staining solution (HBSS containing 1 g ml 1 Hoechst 33342 (62247, Invitrogen, Thermo Fisher Scientific) and either 5 M CellROXTM, 1 M FerroOrange or 1 M BODIPY 581/591 C11)

Vertical bars represent individual genes within each gene set

Its large colony size allows for numerous genotypic replicates through colony fragmentation, while its ability to host symbionts in a facultative manner, has allowed us to pinpoint several key features of gene expression that are important for symbiosis maintenance

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