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The CBS filament adopts a left-handed helical architecture with a twist of 108 and a rise of 51 (Fig

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The activated oocytes were then cultured in bicarbonate-buffered porcine zygote medium-5 (PZM-5) ( in vitro Culture (IVC) medium] and 7.5 mg/mL cytochalasin B for 3 h to suppress the extrusion of the pseudo-second-polar body

At that time, interest in KD for the treatment of this disease decreased and did not come to peoples attention again until the 1990s (2, 3), when its therapeutic effects were gradually found to be effective in a wide range of diseases and more therapeutic potentials of KD were proposed

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Mancini A, Di Segni C, Raimondo S, Olivieri G, Silvestrini A, Meucci E et al (2016) Thyroid hormones, oxidative stress, and inflammation