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Therefore, we summarized the existing LAM detection methods mentioned in the above studies and compared their advantages and disadvantages (Table 3)

doi: 10.1038/s41590-023-01637-4 81 LauR

doi: 10.1371/journal.pone.0057610 122 HanBMengXChenHChenLLiuXWangHet al

On the next day, targeting plasmids (sgRNA or overexpression) were co-transfected with pMD2.G (#12259, Addgene) and psPAX2 (#12260, Addgene) plasmids at 1:1:1 molar ratio using Lipofectamine 2000 (#11668019, Thermo Fisher) at 1:2.5 ratio