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Ion pumps actively transporting ions such as H + , Na + , K + , Ca 2+ and Cu 2+ produce and maintain electrochemical ion gradients across the cellular membranes which are subsequently used by secondary active ion-coupled transporters to deliver molecules in antiport (also known as exchange) or symport (also known as co-transport) of these ions (45, 46)

13B), indicating the robustness of EV-mediated Cas12f delivery

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[DOI] [PMC free article] [PubMed] 152.Moriconi C., Dzieciatkowska M., Roy M., DAlessandro A., Roingeard P., Lee J.Y., Gibb D.R., Tredicine M., McGill M.A., Qiu A., et al