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Some types of unsaturated fatty acids cannot be synthesized in mammalian cells, and so must be consumed as part of the diet, such as omega-3

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Each eye was positioned on a glass plate to obtain the axial length

Carnitine homeostasis, mitochondrial function, and cardiovascular disease

Homogenised tissue was filtered through a 40 m sieve (Corning, Massachusetts, United States), centrifuged at 500 g for 5 min at 4 C to pellet the nuclei and resuspended in buffer supplemented with 1% BSA and 0.2 U/l RNAase inhibitor (Takara Bio, Kusatsu, Japan)

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