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The cells were grown to monolayers in 75-cm 2 culture flasks, and were subsequently harvested with trypsin 0.05%EDTA 0.02% solution and seeded into the 48-well culture plates

doi: 10.1016/j.jhep.2017.11.012 62

Despite the lack of sufficient and solid information on this topic, the following sections attempt to discuss the current evidence supporting hypothetical theoretical frameworks that connect dysbiosis with ME/CFS (see Fig