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Increased lipid peroxidation, a hallmark of ferroptosis, is observed in tubules within IRI kidneys [32, 33], further aligning with the pathological involvement of ferroptosis in renal IRI

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Neutrophils were removed from co-culture with cancer cells after 3 h of incubation at 37 degrees, washed, counted and 50,000 neutrophils re-suspended in 50 l 1X PBS, and placed in a black 96 well plate (Corning 3904), with each condition performed in triplicate

Types There are four main types of vitamin B12 cyanocobalamin, hydroxocobalamin, adenosylcobalamin and methylcobalamin ( All of them can be found in supplements, although cyanocobalamin is the most common

Gene expression analysis was performed via qPCR using the SYBR-Green qPCR Super Mix-UDG kit on a Mastercycler ep realplex detection system (Agilent, USA)